I'm using Ubuntu 14.04.01 in a VirtualBox machine and Unity Tweak Tool works perfectly, everything.
However, after installing Ubuntu in a spare drive (not a virtual machine), Unity Tweak Tool doesn't change themes and font settings (hinting, antialiasing etc.). I haven't tested other settings, but at least it changes panel transparency settings.
Maybe it's something with NVIDIA drivers?
Best Answer
Had same problem after my 14.10 hang up. Solved via:
dconf-editor
org.gnome.settings-daemon.plugins.xsettings
active
checkbox.Or
gsettings set org.gnome.settings-daemon.plugins.xsettings active false
gsettings set org.gnome.settings-daemon.plugins.xsettings active true