IOS – After beta testing your app in TestFlight, do you have to re-upload for public release

The pragmatic way to ensure all testers are off the app is to wait the 30 days for the build to expire and have them manually grab the store version.

You could also upload a new beta build instructing them to do the same. That way they get an email from Apple with the news you want to convey to them and a limited duration to get them all off that build.

No - you'll jam up the review if you don't let the first review complete once it is "In Review". Most of this is covered in the FAQ on iTunes Connect under Resources and Help.

The solution is to dig into the documentation (there's a video explaining the entire test flight process) and/or call Apple Developer support if you can't find the documentation on the web site.

I used to know where the link to support was on developer.apple.com, but Apple recently re-jiggered things on the developer web site and it's not where I expected. I suppose you need to log in to iTunes Connect and get help there on the contact us page.

The main solution I've discovered is to be patient and not try to game the system by submitting a second build if the first is taking a while.

If your build was "In Review" see if you can call and have them resume that review with the original build. Getting that first review is crucial for having subsequent reviews go faster as they can refer to their internal testing notes and just look over what changed, spot check things rather than do all the work for an initial build.